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Genetic Monitoring
Program
Genetic
monitoring is a system for quality control of breeding and
production of laboratory animal. NALC-MU has established
genetic monitoring program that focused on the screening of
genetic integrity in inbred strains and heterogeneity
maintained in outbred stock. The program is composed by
observation and analysis of phenotypic expressions such as
coat color, breeding performance, protein variant and cell
surface antigen.
The routine genetic monitoring items to confirm the accuracy
of the animal strain are performed by
electrophoresis* and
immunological method. The genetic marker items are
presented in table 1**,
2 and 3. According to the program monitoring is
presented in table 4.
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*Electrophoresis
method that be used for allelic pattern checking of
biochemical markers are:
· Cellulose
acetate membrane electrophoresis
(CAME)
·
Polyacrylamide gel electrophoresis (PAGE)
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**Table 1 List of Biochemical marker Items
for Mice |
|
Locus |
Chr. No. |
Gene Name |
Method |
Sample |
|
Idh1 |
1 |
Isocitrate dehydrogenase-1 |
CAME |
Kidney |
|
Pep3 |
1 |
Peptidase-3 |
CAME |
Kidney |
|
Akp1 |
1 |
Alkaline phosphatase-1 |
CAME |
Kidney |
|
Car2 |
3 |
Carbonic anhydrase-2 |
CAME |
Hemolysate |
|
Mup1 |
4 |
Major urinary protein-1 |
PAGE |
Urine |
|
Gpd1 |
4 |
Glucose phosphate dehydrogenase-1 |
CAME |
Kidney |
|
Pgm1 |
5 |
Phosphoglucomutase-1 |
CAME |
Kidney |
|
Ldr1 |
6 |
Lactate dehydrogenase regulator-1 |
CAME |
Hemolysate |
|
Gpi1 |
7 |
Glucose phosphate isomerase-1 |
CAME |
Hemolysate |
|
Hbb |
7 |
Hemoglobin beta chain |
CAME |
Hemolysate |
|
Es1 |
8 |
Esterase-1 |
CAME |
Plasma |
|
Es2 |
8 |
Esterase-2 |
CAME |
Kidney |
|
Mod1 |
9 |
Malic enzyme supernatant |
CAME |
Kidney |
|
Trf |
9 |
Transferrin |
CAME |
Plasma |
|
Es3 |
11 |
Esterase-3 |
CAME |
Kidney |
|
|
Table 2 List
of Biochemical marker Items for Rat |
|
Locus |
Chr. No. |
Gene Name |
Method |
Sample |
|
Hbb |
1 |
Hemoglobin beta chain |
CAME |
Hemolysate |
|
Amy1 |
2 |
Amylase-1 |
CAME |
Pancreas |
|
Svp1 |
3 |
Seminal vesicle protein-1 |
CAME |
Seminal |
|
Acon1 |
5 |
Aconitase-1 |
CAME |
Kidney |
|
Mup1 |
5 |
Major urinary protein-1 |
PAGE |
Urine |
|
Fh1 |
13 |
Fumarate hydratase-1 |
CAME |
Kidney |
|
Gc |
14 |
Group-specific component |
CAME |
Plasma |
|
Es1 |
19 |
Esterase-1 |
PAGE |
Plasma |
|
Es2 |
19 |
Esterase-2 |
PAGE |
Plasma |
|
Es3 |
11 |
Esterase-3 |
PAGE |
Intestine |
|
Es4 |
19 |
Esterase-4 |
CAME |
Kidney |
|
Es14 |
19 |
Esterase-14 |
PAGE |
Plasma |
Genetic Monitoring Report 2009
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