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Health Monitoring

Written by Super User. Posted in HealthMonitoring

          The quality control program of National Laboratory Animal Center, Mahidol University (NLAC-MU) performs a complete investigation of the health status of itslaboratory animals to assure the health quality of  NLAC-MU animals. We promptlyprovide a current Health monitoring report  of our animals  4 times  a year.

 The health monitoring system is divided into two categories :
 

Pathophysiological monitoring
Necropsy Histopathology
Hematology Clinical chemistry
Microbiological monitoring
Item Animals Method
Mouse Rat Hamster Guinea-pig Rabbit
Virus       Serology  
Sialodacryoadenitis virus   x       ELISA, IFA
Sendai virus x x x x x
Mouse hepatitis virus x        
Bacteria Serology & Cultivation  
Mycoplasma pulmonis x x       Culture
ELISA, IFA
Salmonella spp. x x x x x Culture
Citrobacter rodentium x         Culture
Bordetella bronchiseptica   x x x x Culture
Corynebacteriun kutscheri x x       Culture
Pasteurella pneumotropica x x x x x Culture
Pasteurella multocida     x x x Culture
Streptococcus pneumoniae x x x x x Culture
Streptococcus zooepidermicus       x   Culture
Yersinia pseudotuberculosis     x x   Culture
Pseudomonas aeruginosa x x x x x Culture
Staphylococcus aureus x x x x x Culture
Clostridium piliforme x x x x x ELISA, IFA
Fungi         Cultivation  
Dermatophyte x x x x x Culture
Parasite       Microscopy    
Giardia muris x x       Wet mount
Spironucleus muris x x x     Wet mount
Syphacia spp. x x       Wet mount &
Scotch tape
Eimeria x x   x x Floatation
Arthropods x x x x x Scotch tape

                                                                                                                                                                                                                                                                                                                                                                               

 

Hematology

Written by Super User. Posted in HealthMonitoring

Pathophysiological monitoring
NLAC-MU Hematological examination
Test Method*
Hemoglobin (Hb, g/dl)

Erythrocyte (RBC, 106/µl l)

Hematocrit (HCT, %)

Mean Corpuscular Volume (MCV, fl)

Mean Corpuscular Hemoglobin (MCH, g/dl)

Mean Corpuscular Hemoglobin

Concentration (MCHC, g/dl)

Platelets (PLT, 103/µl l)

Leukocyte (WBC, 103/µl)

Neutrophil (N, %)

Lymphocyte (L, %)

Monocyte (M, %)

Eosinophil (E, %)

Basophil (B, %)
Colorimetric Spectrophotometry

Electrical  Impedance Method

Electrical  Impedance Method

Electrical  Impedance Method

Electrical  Impedance Method

Electrical  Impedance Method

Electrical  Impedance Method

Optical Flow Cytometry

Multi Angle Polarized Scatter Separation

Multi Angle Polarized Scatter Separation

Multi Angle Polarized Scatter Separation

Multi Angle Polarized Scatter Separation

Multi Angle Polarized Scatter Separation

Multi Angle Polarized Scatter Separation

* CELL DYN 3700

Hematological data of NLAC-MU Laboratory animals  

 

Clinical Chemistry

Written by Super User. Posted in HealthMonitoring

Pathophysiological monitoring
NLAC-Clinical chemistry examination
List of Clinical chemistry for NLAC-MU laboratory animals
Test Method*
Total protein   (TP,g/dl)

Albumin   (ALB,g/dl)

Total bilirubin   (T-bili,mg/dl)

Alkaline phosphatase   (ALP,U/L)

Aspartate aminotransferase   (AST,U/L)

Alanine aminotransferase   (ALT,U/L)

Blood urea nitrogen   (BUN,mg/dl)

Creatinine   (CREA,mg/dl)

Glucose   (GLU, mg/dl)

Cholesterol   (CHOL,mg/dl)

Triglyceride   (TRG,mg/dl)

Uric acid   (UA,mg/dl)
Biuret

Bromcresol green

DPD

AMP( 2-amino-2-methyl-1-propanol)

IFCC

IFCC

Urease

Jaffe' (rate blanked)

Hexokinase/G-6PDH

CHOD-PAP

GPO-PAP

Enzymatic colorimetric test

* HITACHI 902

Clinical chemistry of NLAC-MU Laboratory animals  

 

Histopathology

Written by Super User. Posted in HealthMonitoring

Pathophysiological monitoring
  
Histopathology
Histopathology examination
Specimen collection 
Fixation
 (10 % Neutral buffer formalin) 
Washing  (Tap water)
Dehydration(Ethyl alcohol and acetone )
Clearing or  Dealcoholization (Xylene)
Infiltration (paraffin)
Embedding (paraffin block)
Sectioning & Attaching  tissue section(4-6 micron)
Staining (H&E Routine Stain)
Mounting (DPX)
Microscopic examination & Interpretation of tissue section by veterinarian
 

 

         

 

HealthMonitoringReport 2017

Written by Super User. Posted in Health Monitoring Report

HeathMonitoring Report 2017

Type Jan. - Mar. Apr. - Jun. Jul. - Sep. Oct. - Dec. 

Maximum barrier facilities

       
  • Mouse : Mlac:ICR
- - - -
  • Mouse : DBA/2Mlac
- - - -
  • Mouse : C57BL/6Mlac
  • Mouse : C57BL/6Mlac-nu
-
  • Mouse : C3H/HeMlac
- - - -
  • Mouse : BALB/cMlac
  • Mouse : BALB/cMlac-nu
  • Mouse : ICR/Mlac
- - - -
  • Mouse : ICR/Mlac-Hydro
-
  • Rat : WMN/Nrs
  • Rat : SHR/Kyo
  • Rat : Mlac:SD
- - - -
  • Rat : Mlac:WR
- - - -
  • Mouse : NOD/Scid
- - - -
Low barrier facilities        
  • Mlac:ICR
  • Mlac:WR
  • Mlac:SD
  • Mlac:DH
-
  • Mlac:NZW
  • Specific pathogens list and category
       
Long panel's serological report 2017

Sentinel SPF Mice

     

          Mouse : MlacICR

     

          Mouse : DBA/2Mlac

     

          Mouse : C57BL/6Mlac

     

          Mouse : C57BL/6Mlac-nu

     

          Mouse : C3H/HeMlac

     

          Mouse : BALB/cMlac

     

          Mouse : BALB/cMlac-nu

     

          Mouse : ICR/Mlac

     

          Mouse : ICR/Mlac-Hydronephrosis

     

          Mouse : NOD/Scid

     

Sentinel SPF Rat

     

          Rat/WMN/Nrs

     

          Rat/SHR/Kyo

     

          Rat : MlacSD