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Quality Control

Written by Super User. Posted in QualityControl

          NLAC- MU Monitoring System includes genetic, health, environment, and biological products quality control were primary to ensure that the animals and biological products are maintained in a standard status.
 
1. Genetic and Health Monitoring

          To assure the quality of our laboratory animals, monitoring system is followed the concepts of ICLAS and FELASA. Our monitoring system has been improved to clarify animal’s genetic quality, and microbiological status with specified list of pathogens and recommended methods which are Serological testing, Cultivation methods, and Microscopic examination. The intensive monitoring program have also been extended to physiological quality control as hematology and blood chemistry  which in terms resulted in a good reference information for biomedical research that uses animals from the Center.

 
2. Environmental Monitoring
          General environmental monitoring includes temperature, humidity, ventilation, and dark/light cycle are routinely checked in each animal facility. Materials and equipments for animals production includes feed, water, bedding, cages, equipment, and air samples are routinely checked for physical and microbiological monitoring
 
3. Biological Product Control
          Biological products includes prepared plated media, animals blood and its product are involved in the quality control system based on hematological, physical appearance, and microbiological check. 

Health Monitoring

Written by Super User. Posted in HealthMonitoring

          The quality control program of National Laboratory Animal Center, Mahidol University (NLAC-MU) performs a complete investigation of the health status of itslaboratory animals to assure the health quality of  NLAC-MU animals. We promptlyprovide a current Health monitoring report  of our animals  4 times  a year.

 The health monitoring system is divided into two categories :
 

Pathophysiological monitoring
Necropsy Histopathology
Hematology Clinical chemistry
Microbiological monitoring
Item Animals Method
Mouse Rat Hamster Guinea-pig Rabbit
Virus       Serology  
Sialodacryoadenitis virus   x       ELISA, IFA
Sendai virus x x x x x
Mouse hepatitis virus x        
Bacteria Serology & Cultivation  
Mycoplasma pulmonis x x       Culture
ELISA, IFA
Salmonella spp. x x x x x Culture
Citrobacter rodentium x         Culture
Bordetella bronchiseptica   x x x x Culture
Corynebacteriun kutscheri x x       Culture
Pasteurella pneumotropica x x x x x Culture
Pasteurella multocida     x x x Culture
Streptococcus pneumoniae x x x x x Culture
Streptococcus zooepidermicus       x   Culture
Yersinia pseudotuberculosis     x x   Culture
Pseudomonas aeruginosa x x x x x Culture
Staphylococcus aureus x x x x x Culture
Clostridium piliforme x x x x x ELISA, IFA
Fungi         Cultivation  
Dermatophyte x x x x x Culture
Parasite       Microscopy    
Giardia muris x x       Wet mount
Spironucleus muris x x x     Wet mount
Syphacia spp. x x       Wet mount &
Scotch tape
Eimeria x x   x x Floatation
Arthropods x x x x x Scotch tape

                                                                                                                                                                                                                                                                                                                                                                               

 

Genetic Monitoring Program

Written by Super User. Posted in GeneticMonitoring

            Genetic monitoring is a system for quality control of breeding and production of laboratory animal. NALC-MU has established genetic monitoring program that focused on the screening of genetic integrity in inbred strains and heterogeneity maintained in outbred stock. The program is composed by observation and analysis of phenotypic expressions such as coat color, breeding performance, protein variant and cell surface antigen.
            The routine genetic monitoring items to confirm the accuracy of the animal strain are performed by electrophoresis* and immunological method. The genetic marker items are presented in table 1**, 2 and 3. According to the program monitoring is presented in table 4.
 
*Electrophoresis method that be used for allelic pattern checking of biochemical markers are :

·  Cellulose acetate membrane electrophoresis (CAME)

· Polyacrylamide gel electrophoresis (PAGE)

 

**Table 1 List of Biochemical marker Items for Mice
Locus Chr.No. Gene Name Method Sample
Idh1 1 Isocitrate dehydrogenase-1 CAME Kidney
Pep3 1 Peptidase-3 CAME Kidney
Akp1 1 Alkaline phosphatase-1 CAME Kidney
Car2 3 Carbonic anhydrase-2 CAME Hemolysate
Mup1 4 Major urinary protein-1 PAGE Urine
Gpd1 4 Glucose phosphate dehy CAME Kidney
Pgm1 5 Phosphoglucomutase-1 CAME Kidney
Ldr1 6 Lactate dehydrogenase regulator-1 CAME Hemolysate
Gpi1 7 Glucose phosphate isomerase-1 CAME Hemolysate
Hbb Hemoglobin beta chain CAME Hemolysate
Es1 8 Esterase-1 CAME Plasma
Es1 8 Esterase-2 CAME Kidney
Mod1 9 Malic enzyme supernatant CAME Kidney
Trf 9 Transferrin CAME Plasma
Es3 11 Esterase-3 CAME Kidney
Table 2 List of Biochemical marker Items for Rat
Locus Chr.No. Gene Name Method Sample
Hbb 1 Hemoglobin beta chain CAME Hemolysate
Amy1 2 Amylase-1 CAME Pancreas
Svp1 3 Seminal vesicle protein-1 CAME Seminal
Acon1 5 Aconitase-1 CAME Kidney
Mup1 5 Major urinary protein-1 PAGE Urine
Fh1 13 Fumarate hydratase-1 CAME Kidney
Gc 14 Group-specific component CAME Plasma
Es1 19 Esterase-1 PAGE Plasma
Es2 19 Esterase-2 PAGE Plasma
Es3 11 Esterase-3 PAGE Intestine
Es4 19 Esterase-4 CAME Kidney
Es14 19 Esterase-14 PAGE Plasma

Genetic Monitoring Report 2009

Histopathology

Written by Super User. Posted in HealthMonitoring

Pathophysiological monitoring
  
Histopathology
Histopathology examination
Specimen collection 
Fixation
 (10 % Neutral buffer formalin) 
Washing  (Tap water)
Dehydration(Ethyl alcohol and acetone )
Clearing or  Dealcoholization (Xylene)
Infiltration (paraffin)
Embedding (paraffin block)
Sectioning & Attaching  tissue section(4-6 micron)
Staining (H&E Routine Stain)
Mounting (DPX)
Microscopic examination & Interpretation of tissue section by veterinarian