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C57BL/6Mlac

Written by Super User. Posted in Animals

Name C57BL/6Mlac
Sciencetific Name Mus  musculus
Strain C57BL/6mlac
Source Central Institute of Experimental Animal; CIEAJapan (1999)
Coat color Black
Biological Data  
Adult body weight 

Male 25-40 grams,Female  20-35 grams.

Average life span 2-3years.
Age at mating

Male 8-9 weeks, Female 8-9 weeks

Estrous cycle  4-5 days
Duration of estrus 6 - 11 hours
Reproductive time 8-12 months
Gestation period  19-22 days
Weaning age  18-21 days
Litter size 1-10 pups (average 7.8)
Normal birth weight  1-2 grams
Post partum estrus  - NO -
Food consumption  3-6 grams /animal/day (Adult)
Water consumption 

8-10 ml/animal/day (Adult)

Growth rate

   Generation 25

Husbandry

Breeding Systems

Two mating systems are applied in breeding C57BL/6Mlac mouse inbred strain. Brother-sister full-sip mating system is used to maintain the strain in foundation stock and traffic light system is used breeding for production.

Foundation Stock

One male and one female of brother-sister are paired permanently throughout their breeding life span. This system allows to record complete breeding data.

Production Stock  

           The harem system or polygamous mating system is used for production animals. One male is housed with 2-4 females and pregnant females are removed to separate cages before parturition. This method increased production of animals.

Environment

The C57BL/6Mlac mouse is housed in controlled environment. The ventilation, temperature and humidity are controlled by HVAC (Heating, Ventilating, Air conditioning) system.  

- Temperature ranged at about 24 ± 2 ํ

- Relative humidity range of about 55 + 10%

- Air changes per hour 10-15 ACH

- Light: dark cycle 12:12 hours

- Mouse is sensitive to noise so the noise level should be maintained at 
< 85 db.

Feed and water

           Mouse is fed on the commercial diet (No. CP 082) product of Perfect companion group co. Ltd., the animal feed manufacturer which milled according to our requested specifications. Food is provided in stainless steel wire- bar lid feeders ad libitum. The RO Water is used for the drinking water which is chlorinated at a concentration of 10-12 ppm to make it free from Pseudomonas aeruginosa. The drinking water is provided in bottles with sipper tubes.

Bedding

 Hard wood chips (or shaving) is used for mouse as bedding material. The same bedding is used in shipping containers. Dried bedding is packed in brown paper bags and sterilized by autoclave at 135 C for 7 minutes before used.

Equipment

Cages used for C57BL/6Mlac
 

Dimensions of Enclosure (W×L×H)(centimeter)

Maximum Number Animals/Enclosure

Types of Enclosure & Description

21.3 × 36.2 × 18.5
(IVC)

6 animals/cage (or) 1 animal (lactating female)/cage

Polysulfonate cages with stainless steel wire-bar lid

19 × 30× 13
(open cge)

6 animals/cage (or) 1 animal (lactating female) / cage

Polycarbonate cages with stainless wire-bar lid